Selected publications from members of the Bone and Joint Mechobiology research group.

2017

Pollard AS, Charlton BG, JR Hutchinson, Gustafsson T, McGonnell I, Timmons JA & Pitsillides AA (2017).  Limb proportions show developmental plasticity in response to embryo movement. Scientific Reports. 7:41926. doi: 10.1038/srep41926.

Suswillo R, Javaheri B, Rawlinson SCF, Dowthwaite GP, Lanyon LE and Pitsillides AA. (2017) Strain uses gap junctions to reverse stimulation of osteoblast proliferation by osteocytes. Cell Biochemistry and Function. 35(1): 56–65.

Miller B, Spevak L, Lukashova L, Javaheri B, Pitsillides AA, Boskey A, Bou-Gharios Carriero A. (2017). Altered bone mechanics, architecture and composition in the skeleton of TIMP-3 deficient mice. Calcified Tissue International. doi: 10.1007/s00223-017-0248-5. [Epub ahead of print] 

Berwick DC, Javaheri B, Wetzel A, Hopkinson M, Nixon-Abell J, Grannó S, Pitsillides AA and Harvey K. (2017) Pathogenic LRRK2 variants are gain-of-function mutations that enhance LRRK2-mediated repression of β-catenin signaling. Molecular Neurodegeneration. 12:9. DOI: 10.1186/s13024-017-0153-4http://rdcu.be/oFPL  

2016 (from 13)

Al-Jazzar A, Javaheri B, Prideaux M, Boyde A, Scudamore CL, Cherifi C, Hay E, Hopkinson M, Boyd M, Cohen-Solal M, Farquharson C, Pitsillides AA. (2016) DMP1 promoter-driven diphtheria toxin receptor transgene expression directs unforeseen effects in multiple tissues. Int J Mol Sci. 18(1). pii: E29. doi: 10.3390/ijms18010029.

Staines KA, Poulet B, Wentworth DN, Pitsillides AA. (2016) The STR/ort mouse model of spontaneous osteoarthritis – an update. Osteoarthritis Cartilage. Dec 11. pii: S1063-4584(16)30478-2.

Javaheri B, Hopkinson M, Poulet B, Pollard AS, Shefelbine SJ, Chang Y, Francis-West P, Bou-Gharios G, Pitsillides AA. (2016) Deficiency and also transgenic overexpression of Timp-3 lead to compromised bone mass and architecture in vivo. PLoS ONE. 11(8):e0159657.

Staines KA, Madi K, Mirczuk SM, Parker S, Burleigh A, Poulet B, Hopkinson M, Bodey AJ, Fowkes RC, Farquharson C, Lee PD, Pitsillides AA. (2016) Endochondral growth defect and deployment of transient chondrocyte behaviours underlie osteoarthritis onset in a natural murine model. Arthritis and Rheumatism. 68(4):880-91. doi: 10.1002/art.39508.

Staines KA, Prideaux M, Buttle DJ, Pitsillides AA, Farquharson C. (2016) E11/podoplanin protein stabilization through inhibition of the proteasome promotes osteocyte differentiation in murine in vitro models. Journal of Cellular Physiology. 231:1392-404. doi: 10.1002/jcp.25282.  

2015 (from 6)

Javaheri B, Carriero A, Staines KA, Chang Y-M, Houston DA, Oldknow KJ, Millan J, Bassir Kazeruni N, Salmon P, Shefelbine S, Farquharson C, Pitsillides AA. (2015). Phospho1 deficiency transiently modifies bone architecture yet produces consistent modification in osteocyte differentiation and vascular porosity with ageing Bone 81:277-91. doi: 10.1016/j.bone.2015.07.035.

Pereira AF, Javaheri b, Pitsillides AA, Shefelbine SJ (2015). Predicting cortical bone adaptation in the mouse tibia. Royal Society Interfac. 12(110):0590. doi: 10.1098/rsif.2015.0590.

Akbareian S, Pitsillides AA, Macharia R, McGonnell I. (2015) Holes in the head: classifying the development of cranial foramina that form in the base of the skull. J Anat. 226(6): 560–574.

Shah M, Gburcik V, Reilly P, Sankey RA, Emery RJ, Clarkin CE, Pitsillides AA. (2015) Local origins impart conserved bone type-related differences in human osteoblast behaviour. Eur Cell Mater. Journal. 29:155-75; discussion 175-6.

Poulet B, de Souza R, Kent AV, Saxon L, Barker O, Wilson A, Chang Y-M, Cake M, Pitsillides AA. (2015). Intermittent applied mechanical loading induces subchondral bone thickening that may be intensified locally by contiguous articular cartilage lesions. Osteoarthritis and Cartilage. 23(6):940-8. doi:10.1016/j.joca.2015.01.012

2014

Poulet B, Souza R, Knight C, Wilson A, Bevan S, Chang Y, Pitsillides AA. (2014) Gait analysis in Str/ort mice reveals modified paw area as a predictor of natural osteoarthritis progression. Arthritis and Rheumatism. 66 (7): 1832–1842.

Carriero A, Abela L, Pitsillides AA and Shefelbine SJ. (2014) Ex vivo determination of bone tissue strains for an in vivo mouse tibial loading model. Journal of Biomechanics 47:2490-7.

2013

Poulet B, Westerhof TA, Hamilton RW, Shefelbine SJ, Pitsillides AA. (2013) Spontaneous osteoarthritis in Str/ort mice is unlikely due to greater vulnerability to mechanical trauma.  Osteoarthritis Cartilage. 21(5):756-63.

2012

Poulet B, Ulici V, Stone TC, Pead M, Gburcik V, Constantinou E, Palmer DB, Beier F, Timmons JA and Pitsillides AA. (2012) Time-series transcriptional profiling yields new perspectives on susceptibility to osteoarthritis. Arthritis and Rheumatism 64(10): 3256-3266.

2011

Hutchinson JR, Delmer C, Miller CE, Hildebrandt T, Pitsillides AA & Boyde A. (2011) From flat foot to fat foot: The structure, ontogeny, function and evolution of elephant “sixth toes”. Science. 334 (6063):1699-703.

Pitsillides AA and Beier F. (2011) Cartilage biology in osteoarthritis--lessons from developmental biology. Nature Reviews Rheumatology. 7(11):654-63

Clarkin CE, Allen S, Kuiper NJ, Wheeler BT, Wheeler-Jones CPD and Pitsillides AA. (2011) Regulation of UDP-glucose dehydrogenase is sufficient to modulate hyaluronan production and release, control sulphated GAG Synthesis and promote chondrogenesis. J Cell Physiol. 226(3):749-61.

Poulet B, Hamilton RW, Shefelbine S and Pitsillides AA. (2011) Characterising a novel and adjustable non-invasive murine knee joint loading model. Arthritis & Rheumatism. 2011 63(1):137-47.

2008-2010

Sztefek P, Vanleene M, Olsson R, Collinson R, Pitsillides AA, Shefelbine S. (2010) Using digital image correlation to determine bone surface strains during loading and after adaptation of the mouse tibia. J Biomech. 43(4):599-605

Rawlinson SCF, Murray DH, Mosley JR, Wright CDP, Bredl JC, Saxon LK, Loveridge N, Leterrier C, Constantin P, Farquharson C and Pitsillides AA. (2009) Genetic selection for fast growth rate generates bone architecture characterised by enhanced periosteal expansion and limited consolidation of the cortices but a diminution in the early responses to mechanical loading. Bone. 45(2): 357–366

Clarkin CE, Emery RJ, Pitsillides AA & Wheeler-Jones CPD (2008) Evaluation of VEGF-mediated signalling in primary human cells reveals a paracrine action for VEGF in osteoblast-mediated crosstalk to endothelial cells. Journal of Cell Physiology. 214:537-44.

2005-2007

Lewthwaite JC, Bastow ER, Lamb KJ, Blenis J, Wheeler-Jones CPD, Pitsillides AA. (2006) A mechano-modulatory role for p38 MAPK in regulating MEK-ERK pathway activation in embryonic joint articular surface cells Journal of Biological Chemistry. 281(16):11011-8.

de Souza, RL, Matsuura M, Eckstein F, Rawlinson SCF, Lanyon L & Pitsillides AA. (2005) Non-invasive model of axial loading of the mouse tibia discloses load-induced increases in cortical bone formation and modification in trabecular bone organisation. Bone 37(6):810-8

Bastow E, Lamb KJ, Osborne A, Kavanagh E, Lewthwaite JC, Wheeler-Jones CPD & Pitsillides AA. (2005). Selective activation of the MEK-ERK pathway is regulated by mechanical stimuli in forming joints and promotes pericellular matrix formation. Journal of Biological Chemistry. 280(12):11749-58.

2002-2004

Hickery M, Bayliss MT, Dudhia J, Lewthwaite JC, Edwards JCW & Pitsillides AA. (2003) Age-related changes in the response of human articular cartilage to rhIL-1a and rhTGFb; chondrocytes exhibit a diminished sensitivity to TGFb. Journal of Biological Chemistry. 278(52):53063-71.

Kavanagh E, Osborne AC, Ashhurst DE & Pitsillides AA. (2002) Keratan sulphate epitopes exhibit a conserved distribution during joint development that remains undisclosed on the basis of GAG charge density. Journal of Histochemistry & Cytochemistry 50(8):1039-47

Loveridge N, Fletcher S, Power J, Caballero-Alias AM, Das-Gupta V, Rushton N, Parker M, Reeve J, Pitsillides AA. (2002) Patterns of osteocytic endothelial nitric oxide synthase expression in the femoral neck cortex: differences between cases of intracapsular hip fracture and controls. Bone 30(6):866-71

1999

Pitsillides AA, Skerry TM & Edwards JCW (1999). The effect of immobilisation on the maintenance of hyaluronan concentrations in diarthrodial joints: alterations in synovial lining cellular phenotype. Rheumatology 38(11): 1108-1112.

Pitsillides AA, Rawlinson SCF, Mosley JR & Lanyon LE. (1999) Genetic selection for enhanced growth at the expense of skeletal adaptability to loading. Journal of Bone & Mineral Research. 14 (6): 980-987.

1995-1998

Pitsillides AA, Rawlinson SCF, Suswillo RFL, Bourrin S, Zaman G & Lanyon LE. (1995) Mechanical strain-induced NO production by bone cells: a possible role in adaptive bone (re)modeling? FASEB J. 9: 1614-1622.

Rawlinson SCF, Mosely JR, Suswillo RFL, Pitsillides AA & Lanyon LE. (1995) Calvarial and limb bone cells in organ and monolayer culture do not show the same early responses to dynamic mechanical strain. Journal of Bone & Mineral Research. 10: 1225-32.

1992-1994

Edwards J, Wilkinson L, Jones M, Soothill P, Henderson K, Worrall J & Pitsillides AA. (1994) Formation of human synovial joint cavities: a possible role for hyaluronan and CD44 in altered joint cohesion. Journal of Anatomy. 185: 355-367.

Pitsillides AA, Will RA, Bayliss MT & Edwards JCW. (1994) Effect of intraarticular corticosteroid on the concentration of hyaluronan in serum and synovial fluid. Arthritis & Rheumatism. 37 (7): 1030-1038.

Pitsillides AA, Wilkinson LS, Mehdizadeh S, Bayliss MT & Edwards JCW. (1993) Uridine diphosphoglucose dehydrogenase in normal and rheumatoid synovium: the description of a specialised synovial lining cell. Journal of Experimental Pathology. 74: 27-34.

Pitsillides AA & Blake SM. (1992) Uridine diphosphoglucose dehydrogenase activity in synovial lining cells in antigen-induced arthritis: an indication of synovial lining cell function. Annals of Rheumatic Diseases. 51: 992-995.

1988-91

Pitsillides AA, Blake SM, Glynn LE, Frost GTB, Bitensky L & Chayen J. (1991) The effect of menadione epoxide on the experimental immune arthritis in the rabbit. Journal of Experimental Pathology. 72: 301-309.

Pitsillides AA, Taylor PM, Bitensky L, Chayen J, Muir IH & Askonas BA. (1988) Rapid changes in target cell lysosomes induced by cytotoxic T-cell: an indication of target cell suicide. European Journal of Immunology. 18: 1203-1208.